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Gene Page - CG Number : CG10149
Gene Summary - CG10149:

CG10149: The gene Regulatory particle non-ATPase 6 is referred to in FlyBase by the symbol Dmel\Rpn6 (CG10149, FBgn0028689). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: endopeptidase activity. There is experimental evidence that it is involved in the biological process: proteolysis. 14 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: PCI/PINT associated module; Proteasome component (PCI) domain; Winged helix-turn-helix transcription repressor DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes.

Gene summary for CG10149 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG10149:CG10149 is perturbed in following parameters in primary screen: Fint2 Fmph2 Okt Tclc Tint1 Tint2 Tint3 Tint4 Tmph1 Tmph2 Tmph3
CG10149 is perturbed in following parameters in classification assay (fluid): LP_Fint1- LP_Fint2- LP_Fint3- NuFluct-(fld) NuSize-(fld) Okt-(fld) Rto1- SP_Fclc- SP_Fint1- SP_Fint2- SP_Fint3-
CG10149 is perturbed in following parameters in classification assay (transferrin): Fclc- Fint1- Fint2- Fint3- Fint4- Fmph1- Fmph2- Fmph3- Fnum- NuCirc+(tfr) NuFluct-(tfr) NuSize-(tfr) Okt-(tfr) Rto1-(tfr) Rto2-(tfr) Rto3-(tfr) Tclc- Tint1- Tint2- Tint3- Tmph1- Tmph2- Tmph3- Tnum-
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG10149.
Cell Count:
CG_NumberCategoryR1R2R3
CG10149Primary screen263617101
CG10149Secondary assay1119608528
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG10149:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
181292-0.6401188183.8959304556.4947075690.4556146130.3623194731.8670627834.1403371110.7232479771.1155548196.430617573.3915088323.3166106071.0603915570.59045643710.495896180.2725836136.576595045.5179560938.7498015923.8063178110.2776989270.188272822-0.717145681
182242.6918332453.7748652772.0408180920.254735092-0.2145150891.6870778194.0443597330.1307832952.19873898910.779038066.9291907862.4188061331.8953448091.29596602522.388405250.95752084912.569647611.3230345913.628809493.810030717-0.749566352.0940639720.638800933
183174-0.882495393.1899568811.2523388590.182354987-1.543508078-2.727553631-1.285115238-3.374297814-2.8792790581.807023199-1.221662072.941980687-1.094469283-2.46442241512.88452159-1.401280152-0.6595725231.297476773-2.922402972-2.829309275-0.8401800350.397444839-0.58753633
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG10149Slide10C0.0040.4200.5400.655
CG10149Slide10O0.0000.5510.3730.452
CG10149Slide10P0.0010.3170.3310.526
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG10149Slide1F0.0000.5390.5460.590
CG10149Slide1O0.0050.8380.8830.809
CG10149Slide1T0.0000.4780.3810.269
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG10149 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
proteolysisGO:0006508inferred from direct assay AND inferred from sequence or structural similarity with HGNC_gene:PSMD11; HGNC:9556; OMIM:604449 AND inferred from sequence or structural similarity with SGD_LOCUS:RPN6; SGD:S0002255
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
proteasome regulatory particle, lid subcomplexGO:0008541non-traceable author statement
proteasome regulatory particle
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
endopeptidase activityGO:0004175inferred from direct assay
Other annotations
DatabaseLink
FlyBaseClick here to see CG10149 in FlyBase
FLIGHTClick here to see CG10149 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG10149 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG10149
Entrez GeneEntrez Gene page for CG10149
UniprotUniprot page for CG10149

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)