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Gene Page - CG Number : CG10223
Gene Summary - CG10223:

CG10223: The gene Topoisomerase 2 is referred to in FlyBase by the symbol Dmel\Top2 (CG10223, FBgn0003732). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA topoisomerase (ATP-hydrolyzing) activity; satellite DNA binding; mRNA binding; rDNA binding; four-way junction DNA binding; DNA binding. There is experimental evidence that it is involved in the biological process: mitotic chromosome condensation; metaphase plate congression; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA topological change; mitosis; mitotic spindle organization; sister chromatid segregation; chromatin silencing. 11 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: ATPase-like, ATP-binding domain; Arginine repressor C-terminal-like domain; DNA topoisomerase II, eukaryotic-type; DNA topoisomerase, type IIA; DNA topoisomerase, type IIA, central; DNA topoisomerase, type IIA, conserved site; DNA topoisomerase, type IIA, subunit A, alpha-helical; DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta; DNA topoisomerase, type IIA, subunit A/C-terminal; DNA topoisomerase, type IIA, subunit B, domain 2; DNA topoisomerase, type IIA, subunit B/N-terminal, alpha-beta; Ribosomal protein S5 domain 2-type fold; Ribosomal protein S5 domain 2-type fold, subgroup; Toprim domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages.

Gene summary for CG10223 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG10223:CG10223 is not perturbed in primary screen.
CG10223 is not tested in classification assay.
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG10223.
Cell Count:
CG_NumberCategoryR1R2R3
CG10223Primary screen493917484
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG10223:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
19141-0.153953365-0.2546620640.0859814351.6634675450.6831064441.1484525411.1211076071.2144884461.924749133-0.311140719-0.0343169680.9054132950.252062229-0.632890189-0.5639595270.569797840.9305341170.5613849341.083578411.875560667-0.206707171-0.348435353-0.255091817
1921851.5827648250.310365977-0.6845399931.7836487880.3984511211.2256187351.1831554461.3137211222.4746782912.6972821043.1723502220.6764674851.2266045380.6093389640.5951940260.3158320522.2353296386.079626773.4617495492.0218292281.1389377362.1760112352.420195421
193950.4184143320.8332574990.969418156-0.4777296471.479246841.8116771122.1081587391.5775140051.166035899-0.034996728-0.05733727-0.546576363-0.634140755-0.156947011.609659634-0.2903964311.0793219240.3641899730.576014751.5328454850.604855678-0.232518058-0.70235833
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):CG10223 is not tested in classification assay
Integrated Annotations for CG10223 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
meiosisGO:0007126traceable author statement
mitosis
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
chromatin accessibility complexGO:0008623inferred from direct assay
insoluble fraction
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
DNA bindingGO:0003677inferred from direct assay
DNA topoisomerase (ATP-hydrolyzing) activity
Other annotations
DatabaseLink
FlyBaseClick here to see CG10223 in FlyBase
FLIGHTClick here to see CG10223 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG10223 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG10223
Entrez GeneEntrez Gene page for CG10223
UniprotUniprot page for CG10223

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)