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Gene Page - CG Number : CG10295
Gene Summary - CG10295:

CG10295: The gene PAK-kinase is referred to in FlyBase by the symbol Dmel\Pak (CG10295, FBgn0014001). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: regulation of cell shape; dorsal closure; regulation of axonogenesis; axon guidance; cell adhesion; regulation of synapse structure and activity; septate junction assembly. 50 alleles are reported. The phenotypes of these alleles are annotated with 16 unique terms, many of which group under: organ system subdivision; organ system; adult segment; multicellular structure; peripheral nervous system; larval head segment; adult antennal lobe; adult; postsynaptic membrane; imaginal precursor. It has 9 annotated transcripts and 9 annotated polypeptides. Protein features are: PAK-box/P21-Rho-binding; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual-specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.

Gene summary for CG10295 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG10295:CG10295 is perturbed in following parameters in primary screen: Fmph2
CG10295 is perturbed in following parameters in classification assay (fluid): CellSize+(fld) LP_Fclc- LP_Fint2+ LP_Fint3+ Okt+(fld) SP_Fclc- SP_Fmph3- SP_Fnum-
CG10295 is not tested in classification assay (transferrin).
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG10295.
Cell Count:
CG10295Primary screen603423396
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG10295:
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse

Integrated Annotations for CG10295 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
cell adhesionGO:0007155inferred from direct assay
axon guidance
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
focal adhesionGO:0005925inferred from direct assay
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
protein serine/threonine kinase activityGO:0004674inferred from sequence or structural similarity
protein serine/threonine kinase activity
Other annotations
FlyBaseClick here to see CG10295 in FlyBase
FLIGHTClick here to see CG10295 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG10295 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG10295
Entrez GeneEntrez Gene page for CG10295
UniprotUniprot page for CG10295

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)