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Gene Page - CG Number : CG10325
Gene Summary - CG10325:

CG10325: The gene abdominal A is referred to in FlyBase by the symbol Dmel\abd-A (CG10325, FBgn0000014). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity. There is experimental evidence for 21 unique biological process terms, many of which group under: anatomical structure development; organ development; biological regulation; multicellular organismal process; regulation of cellular macromolecule biosynthetic process; cellular process involved in reproduction; programmed cell death; gonadal mesoderm development; neuroblast fate commitment; cell fate commitment. 112 alleles are reported. The phenotypes of these alleles are annotated with 109 unique terms, many of which group under: organ system subdivision; organ system; adult segment; thoracic segment; abdominal ventral denticle belt; primordium; abdominal lateral pentascolopidial chordotonal organ lch5; late extended germ band embryo; larval abdominal segment; midgut constriction. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Homeobox protein; Homeobox, conserved site; Homeodomain; Homeodomain, metazoa; Homeodomain-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed during late larval stages, during early pupal stages.

Gene summary for CG10325 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG10325:CG10325 is perturbed in following parameters in primary screen: Fmph2 Okt Tclc Tint1 Tint2 Tint3 Tmph1 Tmph2 Tmph3
CG10325 is perturbed in following parameters in classification assay (fluid): LP_Fclc- LP_Fint1- LP_Fint2- LP_Fint3- LP_Fint4- LP_Fmph1- LP_Fmph2- LP_Fmph3- LP_Fnum- NuCirc-(fld) NuFluct+(fld) Okt+(fld) Rto1- Rto2+ Rto3+ SP_Fclc- SP_Fint1- SP_Fnum-
CG10325 is perturbed in following parameters in classification assay (transferrin): Fclc- Fint1- Fint2- Fint3- Fmph2- Fmph3- Fnum- NuCellD+(tfr) NuCirc-(tfr) NuFluct+(tfr) Okt+(tfr) Rto1-(tfr) Rto2-(tfr) Rto3-(tfr) Tint1+ Tint2+ Tmph2- Tnum-
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG10325.
Cell Count:
CG_NumberCategoryR1R2R3
CG10325Primary screen326241116
CG10325Secondary assay844512703
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG10325:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
191107-0.227077103-0.4223108990.830826014-0.432370056-1.1646522842.9496562364.8975484170.8113121412.3936969457.8633038014.251446864-0.3366562560.829588704-0.2944480994.814734062-0.3612529438.99850377610.7076819917.173352264.3058574373.0918876851.922328850.552455606
1922696.7391813936.2585430912.1608527181.835595541-0.4274604012.7386294073.7920793781.0789280863.7072781973.6680974182.4394958922.1939035346.6563015813.9937054924.244915182.450784245.6846077664.5026664418.6748545423.047808833-0.8485967540.9276647441.811181834
19359-0.286140089-0.099159041-0.3711624071.117834158-1.985288473-1.45820507-0.461317786-1.718964716-1.931245838-0.0038912260.081271975-0.3711522720.313528643-0.063726818-0.823862932-0.969954149-0.339018602-0.132057026-0.863130813-2.077653150.101520326-0.8250410480.571910428
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG10325Slide19C0.0030.6620.7950.693
CG10325Slide19O0.0051.2621.4651.391
CG10325Slide19P0.5400.8540.9990.985
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG10325Slide1F0.0190.8830.8220.815
CG10325Slide1O0.0011.5701.4491.633
CG10325Slide1T0.0170.5460.7400.732
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG10325 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
peripheral nervous system developmentGO:0007422traceable author statement
germ cell migration
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
nucleusGO:0005634non-traceable author statement
nucleus
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
sequence-specific distal enhancer binding RNA polymerase II transcription factor activityGO:0003705inferred from direct assay
sequence-specific DNA binding transcription factor activity
Other annotations
DatabaseLink
FlyBaseClick here to see CG10325 in FlyBase
FLIGHTClick here to see CG10325 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG10325 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG10325
Entrez GeneEntrez Gene page for CG10325
UniprotUniprot page for CG10325

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)