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Gene Page - CG Number : CG10540
Gene Summary - CG10540:

CG10540: The gene capping protein alpha is referred to in FlyBase by the symbol Dmel\cpa (CG10540, FBgn0034577). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: actin binding. There is experimental evidence that it is involved in the biological process: germarium-derived oocyte fate determination; wing disc development; regulation of lamellipodium assembly; actin cytoskeleton organization; negative regulation of actin filament polymerization; cell morphogenesis; actin filament organization; negative regulation of filopodium assembly; phagocytosis, engulfment. 17 alleles are reported. The phenotypes of these alleles are annotated with: eye; wing disc; macrochaeta; wing hinge primordium; wing. It has one annotated transcript and one annotated polypeptide. Protein features are: F-actin capping protein, alpha subunit, conserved site; WASH complex, F-actin capping protein, alpha subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed during early pupal stages.

Gene summary for CG10540 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG10540:CG10540 is perturbed in following parameters in primary screen: Fint2 Fmph2
CG10540 is perturbed in following parameters in classification assay (fluid): LP_Fint1+ NuCellD+(fld) NuCirc+(fld) NuCirc-(fld) NuFluct+(fld) NuSize+(fld) NuSize-(fld)
CG10540 is not tested in classification assay (transferrin).
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG10540.
Cell Count:
CG_NumberCategoryR1R2R3
CG10540Primary screen198759270
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG10540:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
191243-0.903027492-0.3015011990.100040055-0.617758895-1.199884255-0.4136425711.7993813510.202644653-2.4404039210.326681121-0.74475196-0.879478108-1.096265909-0.573526863-0.507244416-1.193325332-2.137220694-2.7339361580.780111605-2.6987724910.375189037.0994420955.49323626
192431.9559207033.3967501622.135647414-0.1463125510.6684275343.4366171184.9662329292.8967070022.3007768550.246842770.6145124820.4357164611.9551757161.526730473-0.4397247590.8160411820.7355925740.7192237350.8767964312.6573068460.2534852282.77657072.728686899
1931933.1142123063.2567084082.988139554-0.1084616541.7179382652.4768171877.1315661121.289540670.552451275-2.332733764-0.6689207890.676587072-1.683314798-1.154626210.249814433-1.858699989-2.210630631-1.086767846-3.4465938830.7232602080.174123384-0.6528266191.51050751
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG10540Slide18C0.3560.9000.9070.827
CG10540Slide18O0.6230.9711.0331.075
CG10540Slide18P0.1620.7880.6740.646
CG10540Slide19C0.9711.0570.9311.006
CG10540Slide19O0.1261.0551.5381.470
CG10540Slide19P0.9900.9710.9521.079
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse

Integrated Annotations for CG10540 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
actin filament organizationGO:0007015inferred from mutant phenotype
barbed-end actin filament capping
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
dynactin complexGO:0005869inferred from sequence or structural similarity
F-actin capping protein complex
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
actin bindingGO:0003779inferred from electronic annotation with InterPro:IPR002189, InterPro:IPR017865
Other annotations
DatabaseLink
FlyBaseClick here to see CG10540 in FlyBase
FLIGHTClick here to see CG10540 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG10540 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG10540
Entrez GeneEntrez Gene page for CG10540
UniprotUniprot page for CG10540

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)