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Gene Page - CG Number : CG1071
Gene Summary - CG1071:

CG1071: The gene E2F transcription factor 2 is referred to in FlyBase by the symbol Dmel\E2f2 (CG1071, FBgn0024371). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; transcription factor binding. There is experimental evidence that it is involved in the biological process: oocyte anterior/posterior axis specification; negative regulation of S phase of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA replication; DNA endoreduplication; oocyte dorsal/ventral axis specification. 27 alleles are reported. The phenotypes of these alleles are annotated with: chorion; eye; follicle cell; ommatidium. It has one annotated transcript and one annotated polypeptide. Protein features are: E2F Family; Transcription factor E2F/dimerisation partner (TDP); Winged helix-turn-helix transcription repressor DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages.

Gene summary for CG1071 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG1071:CG1071 is perturbed in following parameters in primary screen: Fint3
CG1071 is perturbed in following parameters in classification assay (fluid): SP_Fmph1+
CG1071 is perturbed in following parameters in classification assay (transferrin): Fclc+ Fmph1+ Fmph3+ NuCirc-(tfr) Rto1-(tfr) Rto2-(tfr) Rto3-(tfr) Tint1- Tint2- Tint3- Tint4- Tnum+
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG1071.
Cell Count:
CG_NumberCategoryR1R2R3
CG1071Primary screen157580151
CG1071Secondary assay11871000890
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG1071:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG1071Slide20C0.2611.4251.5180.917
CG1071Slide20O0.5460.6900.8021.063
CG1071Slide20P0.1971.1131.2271.008
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG1071Slide1F0.9621.0291.0070.958
CG1071Slide1O0.5861.0841.0740.950
CG1071Slide1T0.0591.1481.4351.239
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG1071 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
negative regulation of transcription, DNA-dependentGO:0045892traceable author statement
negative regulation of DNA replication
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
Myb complexGO:0031523inferred from direct assay
chromatin
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcriptionGO:0001078contributes_to inferred from physical interaction with Rbf2
transcription factor binding
Other annotations
DatabaseLink
FlyBaseClick here to see CG1071 in FlyBase
FLIGHTClick here to see CG1071 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG1071 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG1071
Entrez GeneEntrez Gene page for CG1071
UniprotUniprot page for CG1071

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)