Gene Page - CG Number : CG10850

Gene Summary - CG10850:

CG10850: The gene imaginal discs arrested is referred to in FlyBase by the symbol Dmel\ida (CG10850, FBgn0041147). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: mitotic anaphase-promoting complex activity. There is experimental evidence that it is involved in the biological process: imaginal disc development; positive regulation of mitotic metaphase/anaphase transition; neurogenesis. 13 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: organ system; imaginal precursor; multicellular structure; cell cycle; cellular component organization or biogenesis; cell cycle process; eo support cell; salivary gland imaginal ring; organ system subdivision; sense organ; tracheal system; larval head segment; adult segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Anaphase-promoting complex subunit 5,TPR-containing domain; Tetratricopeptide-like helical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages, in adult female stages.

Gene summary for CG10850 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)

Experimental Summary - CG10850:

CG10850 is perturbed in following parameters in primary screen: Fclc Fmph3 Tclc Tint4
CG10850 is perturbed in following parameters in classification assay (fluid): Rto1- SP_Fint1-
CG10850 is perturbed in following parameters in classification assay (transferrin): Fmph1- Fmph3- Okt+(tfr) Rto1-(tfr) Rto2-(tfr) Rto3-(tfr) Tint4- Tmph1-

Cellular phenotyping(Images):

Click here to access phenotyping images of gene CG10850.

Cell Count:

CG_Number	Category	R1	R2	R3
CG10850	Primary screen	796	940	958
CG10850	Secondary assay	896	905	1063

R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3

Primary screen data - CG10850:

SN	RN	WN	Fint1	Fint2	Fint3	Fint4	Fnum	Fmph1	Fmph2	Fmph3	Fclc	Tint1	Tint2	Tint3	Tint4	Rto1	Rto2	Rto3	Okt	Tnum	Tmph1	Tmph2	Tmph3	Tclc	CellSize
20	1	99	1.549515457	-0.135250695	-0.155542195	0.350786324	0.766125054	1.823953363	1.501155569	1.68241296	1.734312954	1.20220999	3.09283381	0.181405346	0.049372157	0.311147713	1.088156198	1.296774726	1.364059722	2.856039598	0.672027589	2.147779368	0.656438836	2.943913014	1.869851875
20	2	247	0.876123216	-0.162636422	-0.027540401	0.317041862	1.187645191	2.504464921	2.318244296	3.023749709	3.250518124	0.765680856	3.57917785	0.671983071	1.505779547	0.678484559	3.971521086	2.385067253	0.993427756	1.613944631	1.002708001	3.201507508	4.533280575	2.563338582	2.691139503
20	3	37	-0.020000386	0.566714076	0.16851614	1.30008679	1.25188458	3.100123618	1.939123661	3.147598422	4.814420441	2.340276228	4.030893236	-0.201254235	1.739646406	2.653640992	0.432076034	1.341497154	3.332918218	8.65446591	3.916565525	4.741661943	2.975968033	7.672391912	5.071925149

SN: Slide Number; RN: Replicate Number; WN: Well Number

Experimental Data (Classification Assay):

Fluid:

CG_Number	Slide No.	ttest P-value	Ratio 1	Ratio 2	Ratio 3
CG10850	Slide20C	0.983	0.972	1.067	0.955
CG10850	Slide20O	0.657	0.998	1.013	1.149
CG10850	Slide20P	0.340	0.987	0.980	0.817

C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse

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Transferrin:

CG_Number	Slide No.	ttest P-value	Ratio 1	Ratio 2	Ratio 3
CG10850	Slide1F	0.467	1.268	1.166	1.083
CG10850	Slide1O	0.052	1.780	1.561	1.466
CG10850	Slide1T	0.155	0.465	0.524	0.227

F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG10850 :

Gene Ontology Annoations: Biological Process

Biological Process - Term	GO ID	Evidence
mitotic anaphase	GO:0000090	non-traceable author statement imaginal disc development

Gene Ontology Annoations: Cellular Component

Cellular Component - Term	GO ID	Evidence
anaphase-promoting complex	GO:0005680	non-traceable author statement anaphase-promoting complex

Gene Ontology Annoations: Molecular Function

Molecular Function - Term	GO ID	Evidence
binding	GO:0005488	inferred from electronic annotation with InterPro:IPR011990

Other annotations

Database	Link
FlyBase	Click here to see CG10850 in FlyBase
FLIGHT	Click here to see CG10850 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRID	Click here to see CG10850 in BioGRID (Interaction Summary)
Off-target	Click here for Off-target data for CG10850
Entrez Gene	Entrez Gene page for CG10850
Uniprot	Uniprot page for CG10850

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Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)