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Gene Page - CG Number : CG1088
Gene Summary - CG1088:

CG1088: The gene Vacuolar H+-ATPase 26kD E subunit is referred to in FlyBase by the symbol Dmel\Vha26 (CG1088, FBgn0015324). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: proton-transporting ATPase activity, rotational mechanism. It is reported to be involved in the biological process: proton transport. 8 alleles are reported. The phenotype of these alleles is annotated with: Malpighian tubule. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: ATPase, V1/A1 complex, subunit E. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.

Gene summary for CG1088 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG1088:CG1088 is perturbed in following parameters in primary screen: Fclc Fint1 Fint2 Fint3 Fmph1 Fmph2 Fmph3 Tclc
CG1088 is perturbed in following parameters in classification assay (fluid): CellSize-(fld) LP_Fclc- LP_Fint4- LP_Fnum- NuCellD+(fld) NuCirc+(fld) NuSize-(fld) Okt-(fld) Rto1- Rto2- Rto3- SP_Fmph1- SP_Fmph2- SP_Fmph3- SP_Fnum-
CG1088 is perturbed in following parameters in classification assay (transferrin): CellSize-(tfr) Fclc- NuCellD+(tfr) NuFluct+(tfr) Tmph1- Tmph2- Tmph3- Tnum-
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG1088.
Cell Count:
CG_NumberCategoryR1R2R3
CG1088Primary screen460852404
CG1088Secondary assay75412261262
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG1088:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG1088Slide10C0.3621.1451.3300.936
CG1088Slide10O0.0030.7660.6990.621
CG1088Slide10P0.2430.8390.9950.616
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG1088Slide1F0.4311.0360.8870.857
CG1088Slide1O0.2921.2841.1010.983
CG1088Slide1T0.0020.7110.5370.576
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG1088 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
proton transportGO:0015992non-traceable author statement
ATP hydrolysis coupled proton transport
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
vacuolar proton-transporting V-type ATPase, V1 domainGO:0000221inferred from sequence or structural similarity with SGD_LOCUS:VMA4; SGD:S0005859
vacuolar proton-transporting V-type ATPase, V1 domain
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
hydrogen-exporting ATPase activity, phosphorylative mechanismGO:0008553inferred from sequence or structural similarity with SGD_LOCUS:VMA4; SGD:S0005859
hydrogen-exporting ATPase activity, phosphorylative mechanism
Other annotations
DatabaseLink
FlyBaseClick here to see CG1088 in FlyBase
FLIGHTClick here to see CG1088 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG1088 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG1088
Entrez GeneEntrez Gene page for CG1088
UniprotUniprot page for CG1088

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)