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Gene Page - CG Number : CG11246
Gene Summary - CG11246:

CG11246: The gene Rpb8 is referred to in FlyBase by the symbol Dmel\Rpb8 (CG11246, FBgn0037121). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: DNA-directed RNA polymerase activity. There is experimental evidence that it is involved in the biological process: mitotic cell cycle G2/M transition DNA damage checkpoint. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Nucleic acid-binding, OB-fold; Nucleic acid-binding, OB-fold-like; RNA polymerase, Rpb8. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, during early larval stages, in adult female stages.

Gene summary for CG11246 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG11246:CG11246 is perturbed in following parameters in primary screen: Fclc Okt Tclc Tint1 Tint2 Tint3 Tint4 Tmph1 Tmph2 Tmph3
CG11246 is perturbed in following parameters in classification assay (fluid): CellSize-(fld) NuCirc+(fld) NuSize-(fld) Okt-(fld)
CG11246 is perturbed in following parameters in classification assay (transferrin): NuCellD-(tfr) NuCirc+(tfr) NuFluct-(tfr) NuSize-(tfr) Okt-(tfr)
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG11246.
Cell Count:
CG_NumberCategoryR1R2R3
CG11246Primary screen571813511
CG11246Secondary assay9229891151
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG11246:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
2115-0.823571290.3848800750.1872414680.861244343-0.5556253640.7543026191.9005387520.66392266112.6079933121.162803613.7786900435.2322611190.7046398610.61961100434.540223134.01014267919.2980811823.7844880225.9677519811.253396171.4003828210.569510711-0.409131921
212161-0.385831564-0.400195453-0.236696937-0.1967860531.195436992.7059493721.6879464832.8796064754.8947029297.8941647293.4961417440.0083363334.5997357813.84074450829.271399471.9142814939.77623728813.151135989.1783204565.4686500542.740245840.5202514281.005448333
213131-0.698913187-0.1832451390.2615200430.1381491370.330392361.2635377341.2916813481.2805961343.5268385168.7072161994.5526558240.1740264752.9932759992.96203861913.883369790.8840517458.30877090116.093929013.6190910783.906027036-0.9841283440.6958099281.656150377
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG11246Slide10C0.4371.3681.4760.781
CG11246Slide10O0.0530.8490.8790.951
CG11246Slide10P0.7061.3061.4340.601
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG11246Slide2F0.3450.5650.9940.943
CG11246Slide2O0.0660.9160.9430.970
CG11246Slide2T0.3410.5191.1010.746
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG11246 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
transcription from RNA polymerase II promoterGO:0006366inferred from sequence or structural similarity with HGNC_gene:POLR2H; HGNC:9195; OMIM:606023 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB8; SGD:S0005750 AND inferred from sequence or structural similarity with WB:F26F4.11; WP:CE04448
mitotic cell cycle G2/M transition DNA damage checkpoint
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
DNA-directed RNA polymerase II, core complexGO:0005665inferred from sequence or structural similarity with HGNC_gene:POLR2H; HGNC:9195; OMIM:606023 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB8; SGD:S0005750
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
DNA-directed RNA polymerase activityGO:0003899inferred from sequence or structural similarity with HGNC_gene:POLR2H; HGNC:9195; OMIM:606023 AND inferred from sequence or structural similarity with SGD_LOCUS:RPB8; SGD:S0005750 AND inferred from sequence or structural similarity with WB:F26F4.11; WP:CE04448
Other annotations
DatabaseLink
FlyBaseClick here to see CG11246 in FlyBase
FLIGHTClick here to see CG11246 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG11246 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG11246
Entrez GeneEntrez Gene page for CG11246
UniprotUniprot page for CG11246

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)