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Gene Page - CG Number : CG11888
Gene Summary - CG11888:

CG11888: The gene Regulatory particle non-ATPase 2 is referred to in FlyBase by the symbol Dmel\Rpn2 (CG11888, FBgn0028692). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: endopeptidase activity. There is experimental evidence that it is involved in the biological process: response to DNA damage stimulus; proteolysis. 7 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit; Armadillo-like helical; Armadillo-type fold; Proteasome/cyclosome, regulatory subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages.

Gene summary for CG11888 is obtained from FlyBase (FB2013_01, released January 23rd, 2013)
Experimental Summary - CG11888:CG11888 is perturbed in following parameters in primary screen: Fclc Fint1 Fint2 Fint3 Fmph1 Fmph2 Fmph3 Okt Tclc Tint2 Tmph2
CG11888 is perturbed in following parameters in classification assay (fluid): CellSize-(fld) LP_Fclc- LP_Fint1+ LP_Fint2+ LP_Fint3+ LP_Fmph1- LP_Fmph3- LP_Fnum- NuCirc+(fld) NuFluct-(fld) NuSize-(fld) SP_Fclc- SP_Fmph3- SP_Fnum-
CG11888 is perturbed in following parameters in classification assay (transferrin): Fclc- Fint1- Fint2- Fmph2- Fnum- NuCirc+(tfr) NuFluct-(tfr) NuSize-(tfr) Rto1-(tfr) Tclc- Tint1- Tint2- Tmph1- Tmph2- Tmph3- Tnum-
Cellular phenotyping(Images): Click here to access phenotyping images of gene CG11888.
Cell Count:
CG_NumberCategoryR1R2R3
CG11888Primary screen213368187
CG11888Secondary assay10649041208
R1: Replicate No. 1; R2: Replicate No.2; R3: Replicate No. 3
Primary screen data - CG11888:
SNRNWNFint1Fint2Fint3Fint4FnumFmph1Fmph2Fmph3FclcTint1Tint2Tint3Tint4Rto1Rto2Rto3OktTnumTmph1Tmph2Tmph3TclcCellSize
2112893.8565685352.3599364851.5607027582.477053898-0.5191316941.2071544465.8907239021.7311005250.9336489442.3987298290.742771208-0.5992451160.007351868-0.1157553410.36182211-0.8007799360.2761478835.305911674-0.3315855791.5531864470.711753351-0.5976738992.693778534
212276.8952712849.6649141488.1558535474.7122235251.8560523149.15368899210.0055707811.381319177.0484314236.1419525816.058564676-0.7413833990.4165868881.46832261611.675302330.5481202295.3602323518.8156215026.0415989317.030380811-0.6878515552.2074670261.002401484
2131774.1125002577.6279633849.1532993072.2517252632.7808134117.6312832174.6608105999.354621338.5770100881.739532842-0.0572837320.9066490910.2664925790.7722739176.768046158-1.120373594-0.5351793883.5206403090.5779258245.97007723-0.9962024260.437968342-0.308442446
SN: Slide Number; RN: Replicate Number; WN: Well Number
Experimental Data (Classification Assay):Fluid:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG11888Slide11C0.0131.3441.2341.280
CG11888Slide11O0.7531.0411.1480.894
CG11888Slide11P0.7081.0250.8201.388
C=Fdex Chase; O=Surface TfR (Okt9); P=Fdex Pulse


Transferrin:
CG_NumberSlide No.ttest P-valueRatio 1Ratio 2Ratio 3
CG11888Slide2F0.0000.5830.5920.649
CG11888Slide2O0.1100.9240.9410.879
CG11888Slide2T0.0030.6910.5500.705
F=Fdex; O=Surface TfR (Okt9); T=Tf

Integrated Annotations for CG11888 :Gene Ontology Annoations: Biological Process
Biological Process - TermGO IDEvidence
proteolysisGO:0006508inferred from direct assay AND inferred from sequence or structural similarity with HGNC_gene:PSMD1; HGNC:9554; NCBI_NM:NM_002807 AND inferred from sequence or structural similarity with SGD_LOCUS:RPN2; SGD:S0001337
response to DNA damage stimulus
Gene Ontology Annoations: Cellular Component
Cellular Component - TermGO IDEvidence
proteasome regulatory particle, base subcomplexGO:0008540non-traceable author statement
proteasome regulatory particle
Gene Ontology Annoations: Molecular Function
Molecular Function - TermGO IDEvidence
endopeptidase activityGO:0004175inferred from direct assay
enzyme regulator activity
Other annotations
DatabaseLink
FlyBaseClick here to see CG11888 in FlyBase
FLIGHTClick here to see CG11888 in FLIGHT(Compendium of Drosophila in vivo and in vitro RNAi screens)
BioGRIDClick here to see CG11888 in BioGRID (Interaction Summary)
Off-targetClick here for Off-target data for CG11888
Entrez GeneEntrez Gene page for CG11888
UniprotUniprot page for CG11888

Endosite Team :
Prof. Satyajit Mayor (Contact : mayor@ancbs.res.in)
Prof. R. Sowdhamini (Contact : mini@ncbs.res.in)